TIMP1 ELISA 试剂盒
Quick Overview for TIMP1 ELISA 试剂盒 (ABIN2042681)
抗原
See all TIMP1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 3 pg/mL
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组件
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96-well plate pre-coated with anti-TIMP-1, Rat
Lyophilized recombinant TIMP-1, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Rat TIMP-1 in cell culture supernates, serum and plasma (heparin, EDTA).The tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5kD. As a result of glycosylation, the molecular mass is 32-34kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
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别名
- TIMP-1
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基因ID
- 116510
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UniProt
- P30120
抗原 See all TIMP1 ELISA试剂盒
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