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P-Selectin ELISA 试剂盒

This 小鼠 P-Selectin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 P-Selectin.
产品编号 ABIN2042668
发货至: 中国

Quick Overview for P-Selectin ELISA 试剂盒 (ABIN2042668)

抗原

See all P-Selectin (SELP) ELISA试剂盒
P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

适用

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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    62.5 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 5 pg/mL

    组件

    96-well plate pre-coated with anti-sP-Selectin, Mouse
    Lyophilized recombinant sP-Selectin, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse P-Selectin in cell culture supernates, serum and plasma (citrate).P-selectin, also called GMP-140, CD62, or selectin P, is a 140kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1kD. As a result of glycosylation, the molecular mass of 190-200kD is revealed by SDS-PAGE.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all P-Selectin (SELP) ELISA试剂盒

    P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

    别名

    sP-Selectin

    基因ID

    20344

    UniProt

    Q01102
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