P-Selectin ELISA 试剂盒
Quick Overview for P-Selectin ELISA 试剂盒 (ABIN2042667)
抗原
See all P-Selectin (SELP) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 62.5 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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96-well plate pre-coated with anti-sP-Selectin, Human
Lyophilized recombinant sP-Selectin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human P-Selectin in cell culture supernates, serum and plasma (heparin, EDTA, citrate).P-selectin, also called GMP-140, CD62, or selectin P, is a 140kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes.1 It is stored in secretory granules and expressed at the plasma membrane after cell activation.2 It is known to play an important role in atherosclerosis.3 The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).4 The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
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别名
- sP-Selectin
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基因ID
- 6403
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UniProt
- P16109
抗原 See all P-Selectin (SELP) ELISA试剂盒
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