FAP ELISA 试剂盒
Quick Overview for FAP ELISA 试剂盒 (ABIN2042661)
抗原
See all FAP ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 62.5 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-Seprase/FAP, Human
Lyophilized recombinant Seprase/FAP, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Fibroblast Activation Protein, alpha (FAP) ELISA Kit
FAP 适用: 人 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 AA 26-760 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Fibroblast Activation Protein, alpha (FAP) ELISA KitFAP 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human Seprase/FAP in cell culture supernates, serum and plasma (heparin, EDTA).FAP (Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- FAP (Fibroblast Activation Protein, alpha (FAP))
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别名
- Seprase/FAP
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基因ID
- 2191
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UniProt
- Q12884
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途径
- Tube Formation
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