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Soluble E-Selectin ELISA 试剂盒

The 人 Soluble E-Selectin ELISA Kit (ABIN2042658) is a Colorimetric ELISA Kit designed to quantify 人 Soluble E-Selectin.
产品编号 ABIN2042658
发货至: 中国
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Quick Overview for Soluble E-Selectin ELISA 试剂盒 (ABIN2042658)

抗原

See all Soluble E-Selectin (sE-Selectin) products
Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

适用

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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

5-8000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    5 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 4 pg/mL

    组件

    96-well plate pre-coated with anti-sE-Selectin, Human
    Lyophilized recombinant sE-Selectin, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human E-Selectin in cell culture supernates, serum and plasma (heparin, citrate).E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4 The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6kD.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all Soluble E-Selectin (sE-Selectin) products

    Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

    别名

    sE-Selectin

    基因ID

    6401

    UniProt

    P16581
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