CCL5 ELISA 试剂盒
Quick Overview for CCL5 ELISA 试剂盒 (ABIN2042651)
抗原
See all CCL5 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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96-well plate pre-coated with anti-Rantes, Rat
Lyophilized recombinant Rantes, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Rat Rantes in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10kD and, after cleavage of the signal peptide, approximately 8kD. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))
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别名
- Rantes
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基因ID
- 81780
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UniProt
- Q6PED1
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration
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