Osteopontin ELISA 试剂盒
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Quick Overview for Osteopontin ELISA 试剂盒 (ABIN2042625)
抗原
See all Osteopontin (SPP1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6 pg/mL
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-OPN, Mouse
Lyophilized recombinant OPN, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Secreted phosphoprotein 1 (SPP1) ELISA Kit
SPP1 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 小鼠 Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA Milk, Plasma (EDTA), Tissue Culture Medium, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.06 ng/mL - 4 ng/mL Plasma, Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 犬 Colorimetric Sandwich ELISA 0.3125 ng/mL - 10 ng/mL Plasma, Serum, Urine
Secreted phosphoprotein 1 (SPP1) ELISA KitHigh Sensitivity SPP1 适用: 犬 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Secreted phosphoprotein 1 (SPP1) ELISA KitSPP1 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 40 ng/mL Plasma, Serum, Urine
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse OPN in cell culture supernates, serum, plasma (heparin, EDTA) and urine.Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis.1 Osteopontin is presumably involved in stone formation as stone matrix.2 The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4.3 The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5kD. As a result of glycosylation, the molecular mass is 65kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- OPN
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基因ID
- 20750
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UniProt
- Q3UBR1
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途径
- Regulation of Cell Size
抗原 See all Osteopontin (SPP1) ELISA试剂盒
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