MMP13 ELISA 试剂盒
Quick Overview for MMP13 ELISA 试剂盒 (ABIN2042611)
抗原
See all MMP13 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 8 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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96-well plate pre-coated with anti-MMP13, Human
Lyophilized recombinant MMP13, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human MMP13 in cell culture supernates, serum and plasma (heparin).Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene.[1][2]Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene cleaves type II collagen more efficiently than types I and III. [3] The expression of MMP13 in osteoarthritic cartilage and its activity against type II collagen indicates that the enzyme plays a significant role in cartilage collagen degradation and must, therefore, form part of a complex target for proposed therapeutic interventions based on collagenase inhibition. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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别名
- MMP13
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基因ID
- 4322
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UniProt
- A8K846
抗原 See all MMP13 ELISA试剂盒
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