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MMP8 ELISA 试剂盒

This 人 MMP8 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 MMP8.
产品编号 ABIN2042608
发货至: 中国

Quick Overview for MMP8 ELISA 试剂盒 (ABIN2042608)

抗原

See all MMP8 ELISA试剂盒
MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

适用

  • 8
  • 8
  • 7
  • 3
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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

6-10000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    6 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 10 pg/mL

    组件

    96-well plate pre-coated with anti-MMP-8, Human
    Lyophilized recombinant MMP-8, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human MMP-8 in cell culture supernates, serum, plasma (heparin) and saliva.Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells.1 The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein.2 Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.3 The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all MMP8 ELISA试剂盒

    MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

    别名

    MMP-8

    基因ID

    4317

    UniProt

    P22894
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