MMP8 ELISA 试剂盒
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Quick Overview for MMP8 ELISA 试剂盒 (ABIN2042608)
抗原
See all MMP8 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-MMP-8, Human
Lyophilized recombinant MMP-8, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human MMP-8 in cell culture supernates, serum, plasma (heparin) and saliva.Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells.1 The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein.2 Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation.3 The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))
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别名
- MMP-8
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基因ID
- 4317
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UniProt
- P22894
抗原 See all MMP8 ELISA试剂盒
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