Lipocalin 2 ELISA 试剂盒
Quick Overview for Lipocalin 2 ELISA 试剂盒 (ABIN2042579)
抗原
See all Lipocalin 2 (LCN2) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 8 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-Lipocalin-2/NGAL, Mouse
Lyophilized recombinant Lipocalin-2/NGAL, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human Mouse Lipocalin-2/NGAL in cell culture supernates, serum, plasma (heparin) and urine.Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- Lipocalin 2 (LCN2)
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别名
- Lipocalin-2/NGAL
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基因ID
- 16819
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UniProt
- P11672
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途径
- Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis
抗原 See all Lipocalin 2 (LCN2) ELISA试剂盒
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