HAVCR1 ELISA 试剂盒
Quick Overview for HAVCR1 ELISA 试剂盒 (ABIN2042569)
抗原
See all HAVCR1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 2 pg/mL
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组件
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96-well plate pre-coated with anti-KIM1, Mouse
Lyophilized recombinant KIM1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse KIM1 in cell culture supernates, tissue homogenates, serum, plasma (heparin, EDTA) and urine.KIM1 (TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Mouses is encoded by the HAVCR1 gene.[1][2][3] Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led Feigelstock et al. (1998) to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to mouse Tim1, Umetsu et al. (2005) showed that Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the mouse Tim1 gene and database analysis, McIntire et al. (2001) mapped the HAVCR1 gene to 5q33.2. The standard used in this kit is recombinant mouse KIM-1, constituting from Y22-T212 amino acids, and 191amino acids with the molecular mass of 21.8kD.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- HAVCR1 (Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
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别名
- KIM1
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物质类
- Virus
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基因ID
- 171283
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UniProt
- Q3V033
抗原 See all HAVCR1 ELISA试剂盒
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