IL-4 ELISA 试剂盒
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Quick Overview for IL-4 ELISA 试剂盒 (ABIN2042554)
抗原
See all IL-4 (IL4) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 15.6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 1 pg/mL
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组件
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96-well plate pre-coated with anti-IL-4, Rat
Lyophilized recombinant IL-4, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Rat IL-4 in cell culture supernates and serum.Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 Rat IL-4 is a 13.5kD glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- IL-4 (IL4) (Interleukin 4 (IL4))
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别名
- IL-4
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基因ID
- 287287
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UniProt
- P20096
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation
抗原 See all IL-4 (IL4) ELISA试剂盒
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