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HSP27 ELISA 试剂盒

This 人 HSP27 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 HSP27.
产品编号 ABIN2042492
发货至: 中国

Quick Overview for HSP27 ELISA 试剂盒 (ABIN2042492)

抗原

See all HSP27 ELISA试剂盒
HSP27 (Heat Shock Protein 27 (HSP27))

适用

  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5-4000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    62.5 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 5 pg/mL

    组件

    96-well plate pre-coated with anti-HSP27, Human
    Lyophilized recombinant HSP27, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human HSP27 in cell lysates.Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, -crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kD.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all HSP27 ELISA试剂盒

    HSP27 (Heat Shock Protein 27 (HSP27))

    基因ID

    3315

    UniProt

    P04792

    途径

    VEGF Signaling
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