G-CSF ELISA 试剂盒
Quick Overview for G-CSF ELISA 试剂盒 (ABIN2042474)
抗原
See all G-CSF (CSF3) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
-
-
最低检测浓度
- 31.2 pg/mL
-
品牌
- BioAssay™
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 4 pg/mL
-
组件
-
96-well plate pre-coated with anti-G-CSF, Human
Lyophilized recombinant G-CSF, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
-
-
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA Kit
CSF3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 人 Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 兔 Colorimetric Sandwich ELISA 46.875 pg/mL - 3000 pg/mL Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 小鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
Colony Stimulating Factor 3 (Granulocyte) (CSF3) ELISA KitCSF3 适用: 人 Colorimetric Sandwich ELISA 39.06 pg/mL - 2500 pg/mL Plasma, Serum
-
-
板类型
- Pre-coated
-
实验流程
- Sandwich ELISA quantitative detection kit for Human G-CSF in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture.1 G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity.2 Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8kD.
-
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C/-20 °C
-
-
- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
-
别名
- G-CSF
-
基因ID
- 1440
-
UniProt
- P09919
-
途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原 See all G-CSF (CSF3) ELISA试剂盒
-
