CX3CL1 ELISA 试剂盒
Quick Overview for CX3CL1 ELISA 试剂盒 (ABIN2042471)
抗原
See all CX3CL1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 6 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with Eotaxin, MCP-1, MCP-2, 6Ckine, MARC.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-Fractalkine/CX3CL1, Mouse
Lyophilized recombinant Fractalkine/CX3CL1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse Fractalkine/CX3CL1 in cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates.Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a large cytokine protein of 373 amino acids, it contains multiple domains and is the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice).1, 2 CX3CL1 is produced as a long protein (with 373-amino acid in humans) with an extended mucin-like stalk and a chemokine domain on top. The mucin-like stalk permits it to bind to the surface of certain cells. However a soluble (90kD) version of this chemokine has also been observed. Soluble CX3CL1 potently chemoattracts T cells and monocytes, while the cell-bound chemokine promotes strong adhesion of leukocytes to activated endothelial cells, where it is primarily expressed.2 CX3CL1 elicits its adhesive and migratory functions by interacting with the chemokine receptor CX3CR1.3 Its gene is located on human chromosome 16 along with some CC chemokines known as CCL17 and CCL22.2, 4 It can act as a mediator of smooth muscle cell migration in human atherosclerosis, rather than mediate inflammatory cell recruitment.5
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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别名
- Fractalkine/CX3CL1
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基因ID
- 6376
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UniProt
- A0N0N7
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途径
- Synaptic Membrane
抗原 See all CX3CL1 ELISA试剂盒
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