CNTF ELISA 试剂盒
Quick Overview for CNTF ELISA 试剂盒 (ABIN2042409)
抗原
See all CNTF ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 31.2 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-CNTF, Rat
Lyophilized recombinant CNTF, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Rat CNTF in cell culture supernates, serum and plasma (heparin, EDTA, citrate.Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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别名
- CNTF
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基因ID
- 25707
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UniProt
- P20294
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途径
- JAK/STAT Signaling
抗原 See all CNTF ELISA试剂盒
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