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CD25 ELISA 试剂盒

This 小鼠 CD25 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 小鼠 CD25.
产品编号 ABIN2042394
发货至: 中国

Quick Overview for CD25 ELISA 试剂盒 (ABIN2042394)

抗原

See all CD25 (IL2RA) ELISA试剂盒
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

适用

  • 11
  • 7
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小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

8-5000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    8 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 5 pg/mL

    组件

    96-well plate pre-coated with anti-CD25/IL-2sR alpha, Mouse
    Lyophilized recombinant CD25/IL-2sR alpha, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Mouse CD25/IL-2sR alpha in cell culture supernates, serum and plasma (heparin, EDTA).CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all CD25 (IL2RA) ELISA试剂盒

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    别名

    CD25/IL-2sR alpha

    基因ID

    16184

    UniProt

    P01590

    途径

    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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