CD25 ELISA 试剂盒
Quick Overview for CD25 ELISA 试剂盒 (ABIN2042394)
抗原
See all CD25 (IL2RA) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 8 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 5 pg/mL
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组件
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96-well plate pre-coated with anti-CD25/IL-2sR alpha, Mouse
Lyophilized recombinant CD25/IL-2sR alpha, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Mouse CD25/IL-2sR alpha in cell culture supernates, serum and plasma (heparin, EDTA).CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
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别名
- CD25/IL-2sR alpha
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基因ID
- 16184
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UniProt
- P01590
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途径
- JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
抗原 See all CD25 (IL2RA) ELISA试剂盒
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