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CCL22 ELISA 试剂盒

This 人 CCL22 ELISA Kit specifically detects CCL22 .
产品编号 ABIN2042381
发货至: 中国
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Quick Overview for CCL22 ELISA 试剂盒 (ABIN2042381)

抗原

See all CCL22 ELISA试剂盒
CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

适用

  • 12
  • 10
  • 4
  • 3
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  • 2
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检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA
  • 最低检测浓度

    31.2 pg/mL

    交叉反应 (详细)

    There is no detectable cross-reactivity with other relevant proteins.

    灵敏度

    < 1 pg/mL

    组件

    96-well plate pre-coated with anti-CCL22/MDC, Human
    Lyophilized recombinant CCL22/MDC, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • 板类型

    Pre-coated

    实验流程

    Sandwich ELISA quantitative detection kit for Human CCL22/MDC in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage-derived chemokine (MDC), also called Chemokine, cc motif, ligand 22 (CCL22) or Small inducible cytokine subfamily A, member 22 (SCY22). MDC is a recently identified member of the CC chemokine family. It is not closely related to other chemokines, sharing most similarity with thymus- and activation-regulated chemokine (TARC), which contains 37% identical amino acids. In addition, MDC gene is mapped to chromosome 16q13, the same position reported for the TARC gene. MDC has the four-cysteine motif and other highly conserved residues characteristic of CC chemokines, but it shares <35% identity with any of the known chemokines. Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. MDC is highly expressed in macrophages and in monocyte-derived dendritic cells, but not in monocytes, natural killer cells, or several cell lines of epithelial, endothelial, or fibroblast origin. High expression was also detected in normal thymus and less expression in lung and spleen. MDC is thus a unique member of the CC chemokine family that may play a fundamental role in the function of dendritic cells, natural killer cells, and monocytes.

    限制

    仅限研究用
  • 储存条件

    4 °C/-20 °C
  • 抗原 See all CCL22 ELISA试剂盒

    CCL22 (Chemokine (C-C Motif) Ligand 22 (CCL22))

    别名

    CCL22/MDC

    基因ID

    6367

    UniProt

    O00626
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