CD166 ELISA 试剂盒
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Quick Overview for CD166 ELISA 试剂盒 (ABIN2042343)
抗原
See all CD166 (ALCAM) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 2 pg/mL
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交叉反应 (详细)
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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96-well plate pre-coated with anti-ALCAM, Human
Lyophilized recombinant ALCAM, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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板类型
- Pre-coated
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实验流程
- Sandwich ELISA quantitative detection kit for Human ALCAM in cell culture supernates, serum and plasma (heparin, EDTA).CD166 antigen is a 100-105kD typeI transmembrane glycoprotein that is a member of the immunoglobulin superfamily of proteins. In humans it is encoded by the ALCAM gene. The gene was mapped to human chromosome 3q13.1-q13.2 by fluorescence in situ hybridization of cDNA probes to metaphase chromosomes. It is also called CD166 (cluster of differentiation 166), MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. It is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. CD166 protein expression is reported to be upregulated in a cell line deriving from a metastasizing melanoma. CD166 plays an important role in mediating adhesion interactions between thymic epithelial cells and CD6+ cells during intrathymic T cell development. Recently, CD166 has also been used as a potential cancer stem cell marker.
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限制
- 仅限研究用
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储存条件
- 4 °C/-20 °C
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- CD166 (ALCAM) (Activated Leukocyte Cell Adhesion Molecule (ALCAM))
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别名
- ALCAM
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基因ID
- 214
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UniProt
- Q13740
抗原 See all CD166 (ALCAM) ELISA试剂盒
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