Cyclic GMP ELISA 试剂盒
This 化学剂 Cyclic GMP ELISA Kit is a Colorimetric ELISA Kit designed to quantify 化学剂 Cyclic GMP.
Quick Overview for Cyclic GMP ELISA 试剂盒 (ABIN7540317)
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See all Cyclic GMP (cGMP) ELISA试剂盒
Cyclic GMP (cGMP)
适用
化学剂
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
0.3-66.7 pM/mL
应用范围
ELISA
样品类型
Serum, Plasma, Saliva, Cell Culture Supernatant, Urine
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最低检测浓度
- 0.3 pM/mL
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原理
- Quantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.
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Analytical Method
- Quantitative
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特异性
- Easy - use: All reagents and buffers for cGMP test are provided.
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交叉反应 （详细）
- No significant cross-reactivity of similar compounds was found.
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灵敏度
- 0.11 pmol/mL
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产品特性
- cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs). The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.
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组件
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Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cGMP pAb: 12 ml
HRP-cGMP: 6 ml
cGMP Standards (0, 0.3, 0.8, 2.5, 7.4, 22.2, 66.7 pmol/ml): 1.5 ml
cGMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy
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Cyclic GMP (cGMP) ELISA Kit
cGMP 适用: Various Species Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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实验时间
- 2.5 h
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板类型
- Pre-coated
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实验流程
- The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.
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限制
- 仅限研究用
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注意事项
- Do not freeze the kit.
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储存条件
- 4 °C
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储存方法
- The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C.
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有效期
- 12 months
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- Cyclic GMP (cGMP)
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别名
- cGMP
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物质类
- Chemical
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背景
- CGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs).
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