CAPN10 ELISA 试剂盒
This 化学剂 CAPN10 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 化学剂 CAPN10.
Quick Overview for CAPN10 ELISA 试剂盒 (ABIN7540316)
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See all CAPN10 ELISA试剂盒
CAPN10
(Calpain 10 (CAPN10))
适用
化学剂
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
1-243 pM/mL
应用范围
ELISA
样品类型
Serum, Plasma, Saliva, Cell Culture Supernatant, Urine
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最低检测浓度
- 1 pM/mL
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原理
- Quantitative detection of cAMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.
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Analytical Method
- Quantitative
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特异性
- Easy - use: All reagents and buffers for cAMP test are provided.
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交叉反应 （详细）
- No significant cross-reactivity of similar compounds was found
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灵敏度
- 0.61 pmol/mL
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产品特性
- cAMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cAMP (Adenosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases. The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.
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组件
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Anti-IgG Capture Plate: 1 plate (8 wells x 12 strips)
Anti-cAMP mAb: 12 ml
HRP-cAMP: 6 ml
cAMP Standards (0, 1, 3, 9, 27, 81, 243 pmol/ml): 1.5 ml
cAMP Standard Stock (10 nmol/ml): 500 μl
Assay Buffer A: 60 ml
Assay Buffer B: 1 ml
20 × Wash Solution: 40 ml
TMB Substrate: 12 ml
Stop Solution: 6 ml
Plate Sealer: 2 pieces
User Manual: 1 copy
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Calpain 10 (CAPN10) ELISA Kit
CAPN10 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
Calpain 10 (CAPN10) ELISA KitCAPN10 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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实验时间
- 3.5 h
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板类型
- Pre-coated
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实验流程
- The anti-IgG Capture Plate is pre-coated with a fixed amount of Goat anti-mouse IgG to capture Mouse Anti-cAMP Monoclonal Antibody. When free cAMP or specimen and HRP-cAMP are added to the well, they compete in the solution to interact with the cAMP antibody captured on the plate. Other unbound molecules are removed by the wash step. The cAMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the cAMP amount present in the unknown samples can be calculated by transforming its absorbance value.
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限制
- 仅限研究用
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注意事项
- Do not freeze the kit.
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储存条件
- 4 °C
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储存方法
- The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeks at 2-8 °C.
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有效期
- 12 months
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- CAPN10 (Calpain 10 (CAPN10))
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别名
- cAMP / Cyclic AMP
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物质类
- Chemical
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背景
- CAMP is an important secondary messenger in signal transduction pathways that follows a number of extracellular signals. cAMP activates or inhibits various enzymes by promoting their phosphorylation or dephosphorylation. Its concentration is converted from adenosine triphosphate (ATP) via adenylyl cyclases (AC), and is inactivated by hydrolysis to 5'-AMP by the actions of phosphodiesterases.
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途径
- Positive Regulation of Peptide Hormone Secretion
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