CD48 ELISA 试剂盒
Quick Overview for CD48 ELISA 试剂盒 (ABIN1889451)
抗原
See all CD48 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD48
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: F23-S217 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: F23-S217
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CD48 (CD48) ELISA Kit
CD48 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD48 (CD48) ELISA KitCD48 适用: 人 AA 27-220 Colorimetric Sandwich ELISA
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
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板类型
- Pre-coated
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实验流程
- mouse CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD48 has been precoated onto 96-well plates. Standards(NSO, F23-S217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD48 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse CD48 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD48 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 382, Standard deviation: 13.37, CV(%): 3.5
- Sample 2: n=16, Mean(pg/ml): 1554, Standard deviation: 65.27, CV(%): 4.2
- Sample 3: n=16, Mean(pg/ml): 2790, Standard deviation: 131.13, CV(%): 4.7,
- Sample 1: n=24, Mean(pg/ml): 426, Standard deviation: 19.17, CV(%): 4.5
- Sample 2: n=24, Mean(pg/ml): 1713, Standard deviation: 90.79, CV(%): 5.3
- Sample 3: n=24, Mean(pg/ml): 3225, Standard deviation: 187.05, CV(%): 5.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CD48
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别名
- CD48
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背景
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Protein Function: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.
Synonyms: CD48 antigen,BCM1 surface antigen,BLAST-1,HM48-1,MRC OX-45 surface antigen,SLAM family member 2,SLAMF2,Signaling lymphocytic activation molecule 2,sgp-60,CD48,Cd48,Bcm-1,
Full Gene Name: CD48 antigen
Cellular Localisation: Cell membrane, Lipid-anchor, GPI-anchor. -
基因ID
- 12506
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UniProt
- P18181
抗原 See all CD48 ELISA试剂盒
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