DMP1 ELISA 试剂盒

Tissue Specificity: Expressed in tooth particularly in odontoblast, ameloblast and cementoblast. Also expressed in bone particularly in osteoblast.

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse DMP-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse DMP-1 standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 1680, Standard deviation: 70.56, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 4025, Standard deviation: 246, CV(%): 6.1
• Sample 3: n=16, Mean(pg/ml): 6562, Standard deviation: 374, CV(%): 5.7,
• Sample 1: n=24, Mean(pg/ml): 1553, Standard deviation: 89.96, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 3977, Standard deviation: 290, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 6454, Standard deviation: 419.51, CV(%): 6.5

Protein Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity). .

Background: Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein(SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals.

Synonyms: Dentin matrix acidic phosphoprotein 1,DMP-1,Dentin matrix protein 1,AG1,Dmp1,Dmp,

Full Gene Name: Dentin matrix acidic phosphoprotein 1