LGALS7 ELISA 试剂盒
Quick Overview for LGALS7 ELISA 试剂盒 (ABIN1889428)
抗原
See all LGALS7 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Galectin-7
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S2-F136 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: S2-F136
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Galectin 7 (LGALS7) ELISA Kit
LGALS7 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 小鼠 AA 2-136 Colorimetric Sandwich ELISA 93.8-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Galectin 7 (LGALS7) ELISA KitLGALS7 适用: 小鼠 Colorimetric Sandwich ELISA 65-16000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 galectin domain.
Tissue Specificity: Mainly expressed in stratified squamous epithelium.
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板类型
- Pre-coated
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实验流程
- human Galectin-7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Galectin-7 has been precoated onto 96-well plates. Standards(E.coli, S2-F136) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Galectin-7 amount of sample captured in plate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1028, Standard deviation: 48, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 4823, Standard deviation: 255.6, CV(%): 5.3
- Sample 3: n=16, Mean(pg/ml): 6852, Standard deviation: 260.4, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 1580, Standard deviation: 118.5, CV(%): 7.5
- Sample 2: n=24, Mean(pg/ml): 4533, Standard deviation: 389.83, CV(%): 8.6
- Sample 3: n=24, Mean(pg/ml): 6795, Standard deviation: 285.39, CV(%): 4.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- LGALS7 (Galectin 7 (LGALS7))
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别名
- LGALS7
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背景
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Protein Function: Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release. .
Background: Galectin-7, also known as GAL7, is a protein that in humans is encoded by the LGALS7 gene. This gene is mapped to 19q13.2. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Members of this family have been implicated in a variety of functions, including growth regulation, cell adhesion, migration, neoplastic transformation, and immune responses. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It has been found that expression of Galectin-7 was markedly upregulated in corneal epithelium after injury and that exogenous galectin-7 stimulated reepithelialization of corneal wounds.
Synonyms: Galectin-7,Gal-7,HKL-14,PI7,p53-induced gene 1 protein,LGALS7,PIG1,LGALS7B,
Full Gene Name: Galectin-7
Cellular Localisation: Cytoplasm . Nucleus . Secreted . May be secreted by a non-classical secretory pathway. -
基因ID
- 653499
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UniProt
- P47929
抗原 See all LGALS7 ELISA试剂盒
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