CRISP3 ELISA 试剂盒
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- CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))
- 抗原表位
- AA 21-245
- 适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 1.56-100 ng/mL
- 最低检测浓度
- 1.56 ng/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CRISP3
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine, Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: N21-Y245 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <50pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: N21-Y245 - Featured
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Cysteine-Rich Secretory Protein 3 (CRISP3) ELISA Kit
CRISP3 适用: 人 Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cysteine-Rich Secretory Protein 3 (CRISP3) ELISA KitCRISP3 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Tissue Homogenate, Urine
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the CRISP family.
Tissue Specificity: Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
- 板类型
- Pre-coated
- 实验流程
- human CRISP3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRISP3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CRISP3 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(ng/ml): 18, Standard deviation: 0.63, CV(%): 3.5
- Sample 2: n=16, Mean(ng/ml): 41, Standard deviation: 1.7, CV(%): 4.1
- Sample 3: n=16, Mean(ng/ml): 72, Standard deviation: 2.74, CV(%): 3.8,
- Sample 1: n=24, Mean(ng/ml): 20, Standard deviation: 1.14, CV(%): 5.7
- Sample 2: n=24, Mean(ng/ml): 38, Standard deviation: 2.4, CV(%): 6.3
- Sample 3: n=24, Mean(ng/ml): 65, Standard deviation: 3.8, CV(%): 5.8
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CRISP3 ELISA试剂盒
- CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))
- 别名
- CRISP3 (CRISP3 产品)
- 别名
- aln-a ELISA Kit, crisp3 ELISA Kit, MGC76177 ELISA Kit, CRISP3 ELISA Kit, Aeg2 ELISA Kit, CRISP-3 ELISA Kit, CRS3 ELISA Kit, SGP28 ELISA Kit, dJ442L6.3 ELISA Kit, AEG2 ELISA Kit, HSP-3 ELISA Kit, cysteine-rich secretory protein 3 ELISA Kit, cysteine-rich secretory protein 3 S homeolog ELISA Kit, cysteine rich secretory protein 3 ELISA Kit, cysteine-rich secretory protein 2 ELISA Kit, CRISP3 ELISA Kit, crisp3.S ELISA Kit, crisp3 ELISA Kit, CpipJ_CPIJ001507 ELISA Kit, Crisp3 ELISA Kit, LOC481834 ELISA Kit
- 背景
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Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
Synonyms: Cysteine-rich secretory protein 3,CRISP-3,Specific granule protein of 28 kDa,SGP28,CRISP3,
Full Gene Name: Cysteine-rich secretory protein 3
Cellular Localisation: Secreted. In neutrophils, localized in specific granules. - 基因ID
- 10321
- UniProt
- P54108
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