CRISP3 ELISA 试剂盒

Sequence similarities: Belongs to the CRISP family.

Tissue Specificity: Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human CRISP3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human CRISP3 standard solution and each sample is measured in duplicate.

• Sample 1: n=16, Mean(ng/ml): 18, Standard deviation: 0.63, CV(%): 3.5
• Sample 2: n=16, Mean(ng/ml): 41, Standard deviation: 1.7, CV(%): 4.1
• Sample 3: n=16, Mean(ng/ml): 72, Standard deviation: 2.74, CV(%): 3.8,
• Sample 1: n=24, Mean(ng/ml): 20, Standard deviation: 1.14, CV(%): 5.7
• Sample 2: n=24, Mean(ng/ml): 38, Standard deviation: 2.4, CV(%): 6.3
• Sample 3: n=24, Mean(ng/ml): 65, Standard deviation: 3.8, CV(%): 5.8

Background: Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.

Synonyms: Cysteine-rich secretory protein 3,CRISP-3,Specific granule protein of 28 kDa,SGP28,CRISP3,

Full Gene Name: Cysteine-rich secretory protein 3