GNLY ELISA 试剂盒

Tissue Specificity: Expressed in natural killer and T-cells.

Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Granulysin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Granulysin standard solution and each sample be measured in duplicate.

• Sample 1: n=16, Mean(pg/ml): 355, Standard deviation: 20.59, CV(%): 5.8
• Sample 2: n=16, Mean(pg/ml): 862, Standard deviation: 38.79, CV(%): 4.5
• Sample 3: n=16, Mean(pg/ml): 1513, Standard deviation: 65, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 324, Standard deviation: 26.5, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 765, Standard deviation: 55.85, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 1322, Standard deviation: 89.8, CV(%): 6.8

Protein Function: Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.

Background: Granulysin is a substance released by cytotoxic T cells(CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein(SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.

Synonyms: Granulysin,Lymphokine LAG-2,Protein NKG5,T-cell activation protein 519,GNLY,LAG2, NKG5, TLA519,

Full Gene Name: Granulysin