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TFF3 ELISA 试剂盒

TFF3 适用: 人 AA 22-80 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
产品编号 ABIN1889396
发货至: 中国
  • 抗原 See all TFF3 ELISA试剂盒
    TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))
    抗原表位
    AA 22-80
    适用
    • 4
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    15.6-1000 pg/mL
    最低检测浓度
    15.6 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFF3
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: E.coli
    Immunogen sequence: E22-Y80
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: E.coli
    Immunogen sequence: E22-Y80
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 1 P-type (trefoil) domain.

    Tissue Specificity: Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus. Also expressed in the hypothalamus where it is detected in paraventricular, periventricular and supraoptic nuclei (at protein level). .

    板类型
    Pre-coated
    实验流程
    human TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF3 has been precoated onto 96-well plates. Standards(E.coli, E22-Y80) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF3 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human TFF3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFF3 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 164, Standard deviation: 7.38, CV(%): 4.5
    • Sample 2: n=16, Mean(pg/ml): 425, Standard deviation: 22.1, CV(%): 5.2
    • Sample 3: n=16, Mean(pg/ml): 631, Standard deviation: 25.87, CV(%): 4.1,
    • Sample 1: n=24, Mean(pg/ml): 152, Standard deviation: 9.6, CV(%): 6.3
    • Sample 2: n=24, Mean(pg/ml): 395, Standard deviation: 28, CV(%): 7.1
    • Sample 3: n=24, Mean(pg/ml): 640, Standard deviation: 37.76, CV(%): 5.9
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all TFF3 ELISA试剂盒
    TFF3 (Trefoil Factor 3 (Intestinal) (TFF3))
    别名
    TFF3 (TFF3 产品)
    别名
    TFF3 ELISA Kit, ITF ELISA Kit, P1B ELISA Kit, TFI ELISA Kit, mITF ELISA Kit, trefoil factor 3 ELISA Kit, trefoil factor 3, intestinal ELISA Kit, TFF3 ELISA Kit, Tff3 ELISA Kit
    背景

    Protein Function: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). .

    Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

    Synonyms: Trefoil factor 3,Intestinal trefoil factor,hITF,Polypeptide P1.B,hP1.B,TFF3,ITF, TFI,

    Full Gene Name: Trefoil factor 3

    Cellular Localisation: Secreted, extracellular space, extracellular matrix . Cytoplasm.
    基因ID
    7033
    UniProt
    Q07654
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