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Trefoil Factor 2 ELISA 试剂盒

TFF2 适用: 人 AA 24-129 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1889395
发货至: 中国
  • 抗原 See all Trefoil Factor 2 (TFF2) ELISA试剂盒
    Trefoil Factor 2 (TFF2)
    抗原表位
    AA 24-129
    适用
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    7.8-500 pg/mL
    最低检测浓度
    7.8 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TFF2
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: E24-Y129
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <10pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: E24-Y129
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Contains 2 P-type (trefoil) domains.

    Tissue Specificity: Stomach.

    板类型
    Pre-coated
    实验流程
    human TFF2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF2 has been precoated onto 96-well plates. Standards(NSO, E24-Y129) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF2 amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TFF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TFF2 standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 62, Standard deviation: 2.3, CV(%): 3.7
    • Sample 2: n=16, Mean(pg/ml): 126, Standard deviation: 5.3, CV(%): 4.2
    • Sample 3: n=16, Mean(pg/ml): 255, Standard deviation: 13.77, CV(%): 5.4,
    • Sample 1: n=24, Mean(pg/ml): 58, Standard deviation: 2.67, CV(%): 4.6
    • Sample 2: n=24, Mean(pg/ml): 133, Standard deviation: 7.85, CV(%): 5.9
    • Sample 3: n=24, Mean(pg/ml): 263, Standard deviation: 17.62, CV(%): 6.7
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all Trefoil Factor 2 (TFF2) ELISA试剂盒
    Trefoil Factor 2 (TFF2)
    别名
    TFF2 (TFF2 产品)
    别名
    TFF2 ELISA Kit, SML1 ELISA Kit, SP ELISA Kit, PSP ELISA Kit, p4 ELISA Kit, p4-a ELISA Kit, sml1 ELISA Kit, xP4 ELISA Kit, xp4.1 ELISA Kit, mSP ELISA Kit, trefoil factor 2 ELISA Kit, trefoil factor 2 S homeolog ELISA Kit, trefoil factor 2 (spasmolytic protein 1) ELISA Kit, TFF2 ELISA Kit, Tff2 ELISA Kit, tff2.S ELISA Kit
    背景

    Protein Function: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity). .

    Background: Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.

    Synonyms: Trefoil factor 2,Spasmolysin,Spasmolytic polypeptide,SP,TFF2,SML1,

    Full Gene Name: Trefoil factor 2

    Cellular Localisation: Secreted.
    基因ID
    7032
    UniProt
    Q03403
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