CCL9 ELISA 试剂盒
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- 抗原 See all CCL9 (Ccl9) ELISA试剂盒
- CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
- 抗原表位
- AA 22-122
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 7.8-500 pg/mL
- 最低检测浓度
- 7.8 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL9
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: Q22-Q122 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <1pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: Q22-Q122 - Featured
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- Discover our top product Ccl9 ELISA Kit
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Chemokine (C-C Motif) Ligand 9 (Ccl9) ELISA Kit
Ccl9 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 9 (Ccl9) ELISA KitCcl9 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Expressed mainly in the liver, lung, and the thymus, although some expression has been detected in a wide variety of tissues except brain.
- 板类型
- Pre-coated
- 实验流程
- mouse CCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL9 has been precoated onto 96-well plates. Standards(E.coli, Q22-Q122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL9 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse CCL9 standard solution and each sample is measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 34, Standard deviation: 1.19, CV(%): 3.5
- Sample 2: n=16, Mean(pg/ml): 78, Standard deviation: 3.67, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 294, Standard deviation: 14.99, CV(%): 5.1,
- Sample 1: n=24, Mean(pg/ml): 35, Standard deviation: 1.68, CV(%): 4.8
- Sample 2: n=24, Mean(pg/ml): 82, Standard deviation: 4.59, CV(%): 5.6
- Sample 3: n=24, Mean(pg/ml): 302, Standard deviation: 19.03, CV(%): 6.3
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CCL9 (Ccl9) ELISA试剂盒
- CCL9 (Ccl9) (Chemokine (C-C Motif) Ligand 9 (Ccl9))
- 别名
- Ccl9 (Ccl9 产品)
- 别名
- CCF18 ELISA Kit, MRP-2 ELISA Kit, Scya10 ELISA Kit, Scya9 ELISA Kit, chemokine (C-C motif) ligand 9 ELISA Kit, Ccl9 ELISA Kit
- 背景
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Protein Function: Monokine with inflammatory, pyrogenic and chemokinetic properties. Circulates at high concentrations in the blood of healthy animals. Binding to a high-affinity receptor activates calcium release in neutrophils. It also inhibits colony formation of bone marrow myeloid immature progenitors.
Background: Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
Synonyms: C-C motif chemokine 9,CCF18,Macrophage inflammatory protein 1-gamma,MIP-1-gamma,Macrophage inflammatory protein-related protein 2,MRP-2,Small-inducible cytokine A9,CCL9(29-101),CCL9(30-101),CCL9(31-101),Ccl9,Mrp2, Scya10, Scya9,
Full Gene Name: C-C motif chemokine 9
Cellular Localisation: Secreted. - 基因ID
- 20308
- UniProt
- P51670
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