CD56 ELISA 试剂盒
Quick Overview for CD56 ELISA 试剂盒 (ABIN1889387)
抗原
See all CD56 (NCAM1) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 625 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD56/NCAM1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: L20-T711 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <20pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: L20-T711
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Neural Cell Adhesion Molecule 1 (NCAM1) ELISA Kit
NCAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 猴 AA 20-718 Colorimetric Sandwich ELISA
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 人 AA 20-718 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Neural Cell Adhesion Molecule 1 (NCAM1) ELISA KitNCAM1 适用: 大鼠 AA 20-712 Colorimetric Sandwich ELISA
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 2 fibronectin type-III domains.
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板类型
- Pre-coated
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实验流程
- mouse CD56 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD56 has been precoated onto 96-well plates. Standards(NSO, L20-T711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD56 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD56 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL mouse CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD56 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.281, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 17.2, Standard deviation: 0.946, CV(%): 5.5
- Sample 3: n=16, Mean(ng/ml): 28.6, Standard deviation: 1.83, CV(%): 6.4,
- Sample 1: n=24, Mean(ng/ml): 8.3, Standard deviation: 3.984, CV(%): 4.8
- Sample 2: n=24, Mean(ng/ml): 19.5, Standard deviation: 1.326, CV(%): 6.8
- Sample 3: n=24, Mean(ng/ml): 32.5, Standard deviation: 2.34, CV(%): 7.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))
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别名
- NCAM1
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背景
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Protein Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellâ€"cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.
Synonyms: Neural cell adhesion molecule 1,N-CAM-1,NCAM-1,CD56,Ncam1,Ncam,
Full Gene Name: Neural cell adhesion molecule 1
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 17967
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UniProt
- P13595
抗原 See all CD56 (NCAM1) ELISA试剂盒
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