APCS ELISA 试剂盒
Quick Overview for APCS ELISA 试剂盒 (ABIN1889377)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.56 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAP/PTX2
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q21-D224 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <50pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q21-D224
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Amyloid P Component, Serum (APCS) ELISA Kit
APCS 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 200 ng/mL Plasma, Serum, Urine
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 大鼠 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Plasma, Serum
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 大鼠 AA 21-228 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 人 AA 20-223 Colorimetric Sandwich ELISA
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 小鼠 Colorimetric Sandwich ELISA 0.5-32 ng/mL Cell Culture Cells, Plasma, Serum
Amyloid P Component, Serum (APCS) ELISA KitAPCS 适用: 人 Colorimetric Sandwich ELISA 1560-100000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the pentaxin family.
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板类型
- Pre-coated
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实验流程
- mouse SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-D224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAP amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse SAP standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 5.68, Standard deviation: 0.33, CV(%): 5.8
- Sample 2: n=16, Mean(ng/ml): 34, Standard deviation: 1.43, CV(%): 4.2
- Sample 3: n=16, Mean(ng/ml): 61, Standard deviation: 3.05, CV(%): 5,
- Sample 1: n=24, Mean(ng/ml): 5.2, Standard deviation: 0.39, CV(%): 7.5
- Sample 2: n=24, Mean(ng/ml): 39, Standard deviation: 2.11, CV(%): 5.4
- Sample 3: n=24, Mean(ng/ml): 74, Standard deviation: 4.59, CV(%): 6.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- APCS (Amyloid P Component, Serum (APCS))
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别名
- APCS
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背景
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Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Synonyms: Serum amyloid P-component,SAP,Apcs,Ptx2, Sap,
Full Gene Name: Serum amyloid P-component
Cellular Localisation: Secreted. -
UniProt
- P12246
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