CYR61 ELISA 试剂盒
Quick Overview for CYR61 ELISA 试剂盒 (ABIN1889372)
抗原
See all CYR61 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CYR61/CCN1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: A22-D381 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: CHO
Immunogen sequence: A22-D381
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Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA Kit
CYR61 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Saliva, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Cysteine-Rich, Angiogenic Inducer, 61 (CYR61) ELISA KitCYR61 适用: 小鼠 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the CCN family.
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板类型
- Pre-coated
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实验流程
- human CYR61 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CYR61 has been precoated onto 96-well plates. Standards(CHO, A22-D381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CYR61 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYR61 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CYR61 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CYR61 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 628, Standard deviation: 19.5, CV(%): 3.1
- Sample 2: n=16, Mean(pg/ml): 1145, Standard deviation: 36.6, CV(%): 3.2
- Sample 3: n=16, Mean(pg/ml): 1574, Standard deviation: 53.5, CV(%): 3.4,
- Sample 1: n=24, Mean(pg/ml): 570, Standard deviation: 27.93, CV(%): 4.9
- Sample 2: n=24, Mean(pg/ml): 1097, Standard deviation: 58.1, CV(%): 5.3
- Sample 3: n=24, Mean(pg/ml): 1563, Standard deviation: 96.9, CV(%): 6.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))
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别名
- CYR61
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背景
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Protein Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up- regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. .
Background: Cysteine-rich angiogenic inducer 61(CYR61) or CCN family member 1(CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.
Synonyms: Protein CYR61,CCN family member 1,Cysteine-rich angiogenic inducer 61,Insulin-like growth factor-binding protein 10,IBP-10,IGF-binding protein 10,IGFBP-10,Protein GIG1,CYR61,CCN1, GIG1, IGFBP10,
Full Gene Name: Protein CYR61
Cellular Localisation: Secreted. -
基因ID
- 3491
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UniProt
- O00622
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途径
- Positive Regulation of Endopeptidase Activity, Growth Factor Binding
抗原 See all CYR61 ELISA试剂盒
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