TGFBI ELISA 试剂盒
(1 reference)Quick Overview for TGFBI ELISA 试剂盒 (ABIN1889371)
抗原
See all TGFBI ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human TGFBI/betaIG-H3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: G24-H683 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: G24-H683
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Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA Kit
TGFBI 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.63 ng/mL - 40 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 AA 24-683 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 大鼠 AA 24-685 Colorimetric Sandwich ELISA
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: 小鼠 Colorimetric Sandwich ELISA 3 pg/mL-1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Transforming Growth Factor, beta-Induced, 68kDa (TGFBI) ELISA KitTGFBI 适用: Pig Colorimetric Sandwich ELISA 0.937 ng/mL - 60 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Contains 1 EMI domain.
Tissue Specificity: Highly expressed in the corneal epithelium. .
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板类型
- Pre-coated
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实验流程
- human TGFBI ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGFBI has been precoated onto 96-well plates. Standards(NSO, G24-H683) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGFBI is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TGFBI amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human TGFBI standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human TGFBI standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 672, Standard deviation: 30.24, CV(%): 4.5
- Sample 2: n=16, Mean(pg/ml): 1913, Standard deviation: 89.9, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 2844, Standard deviation: 150.7, CV(%): 5.3,
- Sample 1: n=24, Mean(pg/ml): 764, Standard deviation: 39.7, CV(%): 5.2
- Sample 2: n=24, Mean(pg/ml): 1860, Standard deviation: 106.02, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 2927, Standard deviation: 184.4, CV(%): 6.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).
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: "Treg/IL-17 ratio and Treg differentiation in patients with COPD." in: PLoS ONE, Vol. 9, Issue 10, pp. e111044, (2014) (PubMed).
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- TGFBI (Transforming Growth Factor, beta-Induced, 68kDa (TGFBI))
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别名
- TGFBI
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背景
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Protein Function: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
Background: Transforming growth factor, beta-induced, 68 kDa, also known as TGFBI(initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
Synonyms: Transforming growth factor-beta-induced protein ig-h3,Beta ig-h3,Kerato-epithelin,RGD-containing collagen-associated protein,RGD-CAP,TGFBI,BIGH3,
Full Gene Name: Transforming growth factor-beta-induced protein ig-h3
Cellular Localisation: Secreted, extracellular space, extracellular matrix. May be associated both with microfibrils and with the cell surface. -
基因ID
- 7045
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UniProt
- Q15582
抗原 See all TGFBI ELISA试剂盒
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