SFTPD ELISA 试剂盒
Quick Overview for SFTPD ELISA 试剂盒 (ABIN1889358)
抗原
See all SFTPD ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SP-D
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: CHO
Immunogen sequence: A20-F374 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: CHO
Immunogen sequence: A20-F374
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Surfactant Protein D (SFTPD) ELISA Kit
SFTPD 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Bronchoalveolar Lavage, Plasma, Serum, Tissue Homogenate
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 大鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Lung Lavage Fluid, Plasma, Serum
Surfactant Protein D (SFTPD) ELISA KitWide Range SFTPD 适用: 人 Colorimetric Sandwich ELISA 6.25 ng/mL - 400 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 小鼠 Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 大鼠 Colorimetric Sandwich ELISA 78.13 pg/mL - 5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Surfactant Protein D (SFTPD) ELISA KitSFTPD 适用: 人 AA 21-375 Colorimetric Sandwich ELISA 625-40.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the SFTPD family.
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板类型
- Pre-coated
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实验流程
- mouse SP-D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SP-D has been precoated onto 96-well plates. Standards(CHO, A20-F374) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SP-D amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse SP-D standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse SP-D standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 2.2, Standard deviation: 0.099, CV(%): 4.5
- Sample 2: n=16, Mean(ng/ml): 4.6, Standard deviation: 0.268, CV(%): 5.7
- Sample 3: n=16, Mean(ng/ml): 10.5, Standard deviation: 0.672, CV(%): 6.4,
- Sample 1: n=24, Mean(ng/ml): 2.4, Standard deviation: 0.139, CV(%): 5.8
- Sample 2: n=24, Mean(ng/ml): 5.3, Standard deviation: 0.318, CV(%): 6
- Sample 3: n=24, Mean(ng/ml): 12.3, Standard deviation: 0.972, CV(%): 7.9
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- SFTPD (Surfactant Protein D (SFTPD))
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别名
- SFTPD
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背景
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Protein Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.
Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
Synonyms: Pulmonary surfactant-associated protein D,PSP-D,SP-D,Lung surfactant protein D,Sftpd,Sftp4,
Full Gene Name: Pulmonary surfactant-associated protein D
Cellular Localisation: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. -
基因ID
- 20390
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UniProt
- P50404
抗原 See all SFTPD ELISA试剂盒
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