IL17RC ELISA 试剂盒
Quick Overview for IL17RC ELISA 试剂盒 (ABIN1889355)
抗原
See all IL17RC ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-17RC
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: E22-R440 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: E22-R440
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Interleukin 17 Receptor C (IL17RC) ELISA Kit
IL17RC 适用: 人 AA 21-454 Colorimetric Sandwich ELISA 250-16000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17 Receptor C (IL17RC) ELISA KitIL17RC 适用: 人 Colorimetric Sandwich ELISA 0.05-12 ng/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17 Receptor C (IL17RC) ELISA KitIL17RC 适用: 人 Colorimetric Sandwich ELISA 250 pg/mL - 16000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse IL-17RC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-17RC has been precoated onto 96-well plates. Standards(NSO, E22-R440) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17RC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-17RC amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IL-17RC standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-17RC standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1458, Standard deviation: 61.24, CV(%): 4.2
- Sample 2: n=16, Mean(pg/ml): 4112, Standard deviation: 267.3, CV(%): 6.5
- Sample 3: n=16, Mean(pg/ml): 6822, Standard deviation: 388.9, CV(%): 5.7,
- Sample 1: n=24, Mean(pg/ml): 1632, Standard deviation: 88.13, CV(%): 5.4
- Sample 2: n=24, Mean(pg/ml): 4473, Standard deviation: 335.5, CV(%): 7.5
- Sample 3: n=24, Mean(pg/ml): 7188, Standard deviation: 488.8, CV(%): 6.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IL17RC (Interleukin 17 Receptor C (IL17RC))
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别名
- IL17RC
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背景
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Background: Interleukin-17 receptor C is a protein that in humans is encoded by the IL17RC gene. By genomic sequence analysis, IL17RC gene was mapped to chromosome 3p25.3-p24.1. This gene encodes a single-pass transmembrane protein that shares limited similarity with the interleukin-17 receptor. Multiple alternatively spliced transcript variants encoding different isoforms have been detected for this gene, but the full-length nature of only three have been determined to date. The biologic activity of IL-17 is dependent on a complex composed of IL-17RA and IL-17RC, suggesting a new paradigm for understanding the interactions between the expanded family of IL-17 ligands and their receptors.
Synonyms: Interleukin-17 receptor C,IL-17 receptor C,IL-17RC,Interleukin-17 receptor-like protein,IL-17RL,ZcytoR14,Il17rc,
Full Gene Name: Interleukin-17 receptor C
Cellular Localisation: Membrane, Single-pass type I membrane protein. -
基因ID
- 171095
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UniProt
- Q8K4C2
抗原 See all IL17RC ELISA试剂盒
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