N-Cadherin ELISA 试剂盒
Quick Overview for N-Cadherin ELISA 试剂盒 (ABIN1889345)
抗原
See all N-Cadherin (CDH2) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Cadherin-2/N-Cadherin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: D160-A724 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <20pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: D160-A724
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Cadherin 2 (CDH2) ELISA Kit
CDH2 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 大鼠 AA 160-724 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 人 AA 160-724 Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 猴 AA 160-724 Colorimetric Sandwich ELISA
Cadherin 2 (CDH2) ELISA KitCDH2 适用: Pig AA 160-724 Colorimetric Sandwich ELISA
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 犬 AA 160-724 Colorimetric Sandwich ELISA
Cadherin 2 (CDH2) ELISA KitCDH2 适用: 兔 AA 160-724 Colorimetric Sandwich ELISA
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse Cadherin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Cadherin-2 has been precoated onto 96-well plates. Standards(NSO, D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Cadherin-2 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Cadherin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Cadherin-2 standard solution and each sample is measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 14.5, Standard deviation: 0.609, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 31.8, Standard deviation: 1.24, CV(%): 3.9
- Sample 3: n=16, Mean(ng/ml): 41.2, Standard deviation: 2.11, CV(%): 5.1,
- Sample 1: n=24, Mean(ng/ml): 15.5, Standard deviation: 0.899, CV(%): 5.8
- Sample 2: n=24, Mean(ng/ml): 34.2, Standard deviation: 1.78, CV(%): 5.2
- Sample 3: n=24, Mean(ng/ml): 42.5, Standard deviation: 2.72, CV(%): 6.4
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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别名
- CDH2
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背景
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Protein Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463). CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. .
Background: Cadherin-2(CDH2), also known as neural cadherin(NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
Synonyms: Cadherin-2,Neural cadherin,N-cadherin,CD325,Cdh2,
Full Gene Name: Cadherin-2
Cellular Localisation: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 12558
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UniProt
- P15116
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途径
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
抗原 See all N-Cadherin (CDH2) ELISA试剂盒
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