CCL25 ELISA 试剂盒
Quick Overview for CCL25 ELISA 试剂盒 (ABIN1889341)
抗原
See all CCL25 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- For quantitative detection of human CCL25 in cell culture supernates, serum and plasma(heparin, EDTA).
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
- Natural and recombinant human CCL25
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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组件
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- 96-well plate precoated with anti- human CCL25 antibod - 1
- Lyophilized recombinant human CCL25 standar - 10ng/tubex2
- Biotinylated anti- human CCL25 antibod - 130μl(dilution 1:100)
- Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
- Sample diluent buffe - 30ml
- Antibody diluent buffe - 12ml
- ABC diluent buffe - 12ml
- TMB color developing agen - 10ml
- TMB stop solutio - 10ml
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: Q24-L150
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Chemokine (C-C Motif) Ligand 25 (CCL25) ELISA Kit
CCL25 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 25 (CCL25) ELISA KitCCL25 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 25 (CCL25) ELISA KitCCL25 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 25 (CCL25) ELISA KitSmall Sample CCL25 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 25 (CCL25) ELISA KitCCL25 适用: 小鼠 AA 24-144 Colorimetric Sandwich ELISA
Chemokine (C-C Motif) Ligand 25 (CCL25) ELISA KitCCL25 适用: 小鼠 Colorimetric Sandwich ELISA 15-2000 pg/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- human CCL25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL25 has been precoated onto 96-well plates. Standards(E.coli, Q24-L150) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL25 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL25 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human CCL25 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 423, Standard deviation: 15.23, CV(%): 3.6
- Sample 2: n=16, Mean(pg/ml): 1094, Standard deviation: 49.23, CV(%): 4.5
- Sample 3: n=16, Mean(pg/ml): 2516, Standard deviation: 158.51, CV(%): 6.3,
- Sample 1: n=24, Mean(pg/ml): 461, Standard deviation: 28.12, CV(%): 6.1
- Sample 2: n=24, Mean(pg/ml): 1273, Standard deviation: 85.29, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 2338, Standard deviation: 168.34, CV(%): 7.2
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- CCL25 (Chemokine (C-C Motif) Ligand 25 (CCL25))
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别名
- CCL25
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背景
- Chemokine(C-C motif) ligand 25(CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK(Thymus-Expressed Chemokine). The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9
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基因ID
- 6370
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UniProt
- O15444
抗原 See all CCL25 ELISA试剂盒
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