PON1 ELISA 试剂盒
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- 抗原 See all PON1 ELISA试剂盒
- PON1 (Paraoxonase 1 (PON1))
- 抗原表位
- AA 2-355
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human PON1
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Tissue Homogenate
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: NSO
Immunogen sequence: A2-L355 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: NSO
Immunogen sequence: A2-L355 - Featured
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Paraoxonase 1 (PON1) ELISA Kit
PON1 适用: 小鼠 Colorimetric Sandwich ELISA 12.5 ng/mL - 800 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Paraoxonase 1 (PON1) ELISA KitPON1 适用: 人 Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Paraoxonase 1 (PON1) ELISA KitPON1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Paraoxonase 1 (PON1) ELISA KitPON1 适用: 小鼠 AA 30-355 Colorimetric Sandwich ELISA
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Sequence similarities: Belongs to the paraoxonase family.
Tissue Specificity: Plasma, associated with HDL (at protein level). Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. .
- 板类型
- Pre-coated
- 实验流程
- human PON1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for PON1 has been precoated onto 96-well plates. Standards(NSO, A2-L355) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PON1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human PON1 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PON1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin) or tissue homogenates to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human PON1 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 289, Standard deviation: 18.8, CV(%): 6.5
- Sample 2: n=16, Mean(pg/ml): 612, Standard deviation: 31.2, CV(%): 5.1
- Sample 3: n=16, Mean(pg/ml): 935, Standard deviation: 50.49, CV(%): 5.4,
- Sample 1: n=24, Mean(pg/ml): 260, Standard deviation: 19.24, CV(%): 7.4
- Sample 2: n=24, Mean(pg/ml): 712, Standard deviation: 46.28, CV(%): 6.5
- Sample 3: n=24, Mean(pg/ml): 1284, Standard deviation: 86.03, CV(%): 6.7
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
-
- 抗原 See all PON1 ELISA试剂盒
- PON1 (Paraoxonase 1 (PON1))
- 别名
- PON1 (PON1 产品)
- 别名
- esa ELISA Kit, pon ELISA Kit, pon2 ELISA Kit, MGC53915 ELISA Kit, MGC89222 ELISA Kit, PON1 ELISA Kit, ESA ELISA Kit, MVCD5 ELISA Kit, PON ELISA Kit, Pon ELISA Kit, paraoxonase 2 ELISA Kit, paraoxonase 1 ELISA Kit, pon2 ELISA Kit, PON1 ELISA Kit, Pon1 ELISA Kit
- 背景
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Protein Function: Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. .
Background: Serum paraoxonase/arylesterase 1(PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1(paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein(HDL).
Synonyms: Serum paraoxonase/arylesterase 1,PON 1,3.1.1.2,3.1.1.81,3.1.8.1,Aromatic esterase 1,A-esterase 1,K-45,Serum aryldialkylphosphatase 1,PON1,PON,
Full Gene Name: Serum paraoxonase/arylesterase 1
Cellular Localisation: Secreted, extracellular space. - 基因ID
- 5444
- UniProt
- P27169
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