TGFB3 ELISA 试剂盒
Quick Overview for TGFB3 ELISA 试剂盒 (ABIN1889335)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Mouse TGF-beta 3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: sf21
Immunogen sequence: A301-S412 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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预测反应
- Bovine,Canine,Chicken,Horse,Monkey,Rabbit
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: sf21
Immunogen sequence: A301-S412
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Transforming Growth Factor, beta 3 (TGFB3) ELISA Kit
TGFB3 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 兔 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: Pig Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 马 Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitSmall Sample TGFB3 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 人 Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 大鼠 AA 301-412 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum, Urine
Transforming Growth Factor, beta 3 (TGFB3) ELISA KitTGFB3 适用: 马 AA 301-412 Colorimetric Sandwich ELISA
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Tissue Specificity: Expressed in both testis and ovary.
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板类型
- Pre-coated
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实验流程
- mouse TGF-beta 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TGF-beta 3 has been precoated onto 96-well plates. Standards(sf21, A301-S412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TGF-beta 3 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TGF-beta 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated mouse cell culture supernates, serum, plasma(EDTA) or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse TGF-beta 3 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 158, Standard deviation: 7.43, CV(%): 4.7
- Sample 2: n=16, Mean(pg/ml): 476, Standard deviation: 20.5, CV(%): 4.3
- Sample 3: n=16, Mean(pg/ml): 935, Standard deviation: 50.5, CV(%): 5.4,
- Sample 1: n=24, Mean(pg/ml): 182, Standard deviation: 10.56, CV(%): 5.8
- Sample 2: n=24, Mean(pg/ml): 563, Standard deviation: 32.1, CV(%): 5.7
- Sample 3: n=24, Mean(pg/ml): 1138, Standard deviation: 74, CV(%): 6.5
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))
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别名
- TGFB3
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背景
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Protein Function: Involved in embryogenesis and cell differentiation.
Background: Transforming growth factor beta 3(TGF-beta 3) is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily. TGF-beta 3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix(ECM) formation during the process of palate development. Without TGF-beta 3, mammals develop a deformity known as acleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta 3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin. TGF-beta 3 activated Lef1 in the absence of beta-catenin(CTNNB1) via nuclear phospho-Smad2 and Smad4.
Synonyms: Transforming growth factor beta-3,TGF-beta-3,Latency-associated peptide,LAP,Tgfb3,
Full Gene Name: Transforming growth factor beta-3
Cellular Localisation: Secreted. -
基因ID
- 21809
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UniProt
- P17125
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途径
- Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus
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