我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Periostin ELISA 试剂盒

Name: Periostin ELISA 试剂盒
SKU: ABIN1889333
Availability: OutOfStock

This 人 Periostin ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Periostin. This ELISA Kit has been cited in 2+ publications.

ELISA

1 image

(2 references)

产品编号 ABIN1889333

发货至: 中国

Quick Overview for Periostin ELISA 试剂盒 (ABIN1889333)

抗原

See all Periostin (POSTN) ELISA试剂盒

Periostin (POSTN)

抗原表位

AA 22-836

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

156-10000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Discover our top product POSTN ELISA Kit

最低检测浓度

156 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Periostin/OSF2

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: N22-Q836

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: N22-Q836
Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum

Periostin (POSTN) ELISA Kit
POSTN 适用: 小鼠 Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum

Periostin (POSTN) ELISA Kit
POSTN 适用: 大鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate

Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Periostin (POSTN) ELISA Kit
POSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate

Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 3.13 ng/mL - 200 ng/mL Cell Culture Supernatant, Plasma, Serum

Periostin (POSTN) ELISA Kit
POSTN 适用: 大鼠 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum

Periostin (POSTN) ELISA Kit
POSTN 适用: 小鼠 AA 24-836 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Periostin (POSTN) ELISA Kit
POSTN 适用: 小鼠 Colorimetric Sandwich ELISA 0.05-16 ng/mL Cell Culture Supernatant, Plasma, Serum

Periostin (POSTN) ELISA Kit
POSTN 适用: 人 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Sequence similarities: Contains 1 EMI domain.
Tissue Specificity: Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. .

板类型

Pre-coated

实验流程

human Periostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Periostin has been precoated onto 96-well plates. Standards(NSO, N22-Q836) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Periostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Periostin amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Periostin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Periostin standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 1392, Standard deviation: 90.48, CV(%): 6.5
- Sample 2: n=16, Mean(pg/ml): 2593, Standard deviation: 121.9, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 4831, Standard deviation: 280.2, CV(%): 5.8,
- Sample 1: n=24, Mean(pg/ml): 1436, Standard deviation: 107.7, CV(%): 7.5
- Sample 2: n=24, Mean(pg/ml): 2734, Standard deviation: 153, CV(%): 5.6
- Sample 3: n=24, Mean(pg/ml): 5166, Standard deviation: 330.6, CV(%): 6.4
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Qin, Yan, Zhang, Wang, Shen, Lv, Li, Wei, Chen: "TGFβ3-mediated induction of Periostin facilitates head and neck cancer growth and is associated with metastasis." in: Scientific reports, Vol. 6, pp. 20587, (2016) (PubMed).

Krencik, Hokanson, Narayan, Dvornik, Rooney, Rauen, Weiss, Rowitch, Ullian: "Dysregulation of astrocyte extracellular signaling in Costello syndrome." in: Science translational medicine, Vol. 7, Issue 286, pp. 286ra66, (2015) (PubMed).
抗原 See all Periostin (POSTN) ELISA试剂盒

Periostin (POSTN)

别名

POSTN

背景

Protein Function: Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity). Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. .
Background: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
Synonyms: Periostin,PN,Osteoblast-specific factor 2,OSF-2,POSTN,OSF2,
Full Gene Name: Periostin
Cellular Localisation: Golgi apparatus . Secreted, extracellular space, extracellular matrix . Colocalizes with BMP1 in the Golgi..

基因ID

10631

UniProt

Q15063