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TGFB2 ELISA 试剂盒

Name: TGFB2 ELISA 试剂盒
SKU: ABIN1889331
Availability: OutOfStock

This 大鼠 TGFB2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 大鼠 TGFB2. This ELISA Kit has been cited in1 publication.

ELISA

1 image

(1 reference)

产品编号 ABIN1889331

发货至: 中国

Quick Overview for TGFB2 ELISA 试剂盒 (ABIN1889331)

抗原

See all TGFB2 ELISA试剂盒

TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

抗原表位

AA 303-414

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

31.2-2000 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Discover our top product TGFB2 ELISA Kit

最低检测浓度

31.2 pg/mL

原理

Sandwich High Sensitivity ELISA kit for Quantitative Detection of activated Rat TGF-beta 2

品牌

PicoKine™

Analytical Method

Quantitative

特异性

Expression system for standard: NSO
Immunogen sequence: A303-S414

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

<10pg/mL

试剂未包括

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

免疫原

Expression system for standard: NSO
Immunogen sequence: A303-S414
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Transforming Growth Factor, beta 2 (TGFB2) ELISA Kit
TGFB2 适用: 小鼠 AA 303-414 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

说明

Tissue Specificity: Isoform TGF-beta2B expressed in the aorta, primary bronchus, uterus, heart, skeletal muscle, sciatic nerve and spinal cord but not in the intestine. .

板类型

Pre-coated

实验流程

rat TGF-beta 2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TGF-beta 2 has been precoated onto 96-well plates. Standards(NSO, A303-S414) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TGF-beta 2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat TGF-beta 2 amount of sample captured in plate.

实验流程

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat TGF-beta 2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of activated rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat TGF-beta 2 standard solution and each sample be measured in duplicate.

实验精密度
- Sample 1: n=16, Mean(pg/ml): 304, Standard deviation: 16.42, CV(%): 5.4
- Sample 2: n=16, Mean(pg/ml): 614, Standard deviation: 31.93, CV(%): 5.2
- Sample 3: n=16, Mean(pg/ml): 1172, Standard deviation: 79.7, CV(%): 6.8,
- Sample 1: n=24, Mean(pg/ml): 377, Standard deviation: 23.75, CV(%): 6.3
- Sample 2: n=24, Mean(pg/ml): 536, Standard deviation: 39.13, CV(%): 7.3
- Sample 3: n=24, Mean(pg/ml): 1223, Standard deviation: 88.1, CV(%): 7.2
限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

有效期

12 months
Li, Liu, Shen, Wang, Chen, Xu, Wen: "MicroRNA-26a modulates transforming growth factor beta-1-induced proliferation in human fetal lung fibroblasts." in: Biochemical and biophysical research communications, Vol. 454, Issue 4, pp. 512-517, (2014) (PubMed).
抗原 See all TGFB2 ELISA试剂盒

TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

别名

TGFB2

背景

Protein Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
Background: Transforming growth factor-beta 2(TGF-beta 2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. This gene is mapped to 1q41. It is an extracellular glycosylated protein. It is known to suppress the effects of interleukin dependent T-cell tumors. TGF-beta 2 is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma(POAG). Studies have shown that TGF-beta 2 influences cultured trabecular meshwork cells, and it reduced outflow facility when perfused into cultured human anterior segments. In POAG, elevated expression of Gremlin by TM cells inhibited BMP4 antagonism of TGF-beta 2 and led to increased extracellular matrix deposition and elevated IOP.
Synonyms: Transforming growth factor beta-2,TGF-beta-2,Latency-associated peptide,LAP,Tgfb2,
Full Gene Name: Transforming growth factor beta-2
Cellular Localisation: Secreted.

基因ID

81809

UniProt

Q07257

途径

Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus