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NOV ELISA 试剂盒

NOV 适用: 人 AA 32-357 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
产品编号 ABIN1889323
发货至: 中国
  • 抗原 See all NOV ELISA试剂盒
    NOV (Nephroblastoma Overexpressed (NOV))
    抗原表位
    AA 32-357
    适用
    • 4
    • 4
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    31.2-2000 pg/mL
    最低检测浓度
    31.2 pg/mL
    应用范围
    ELISA
    原理
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NOV/CCN3
    品牌
    PicoKine™
    样品类型
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    特异性
    Expression system for standard: NSO
    Immunogen sequence: T32-M357
    交叉反应 (详细)
    There is no detectable cross-reactivity with other relevant proteins.
    灵敏度
    <2pg/mL
    试剂未包括
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    免疫原
    Expression system for standard: NSO
    Immunogen sequence: T32-M357
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  • 应用备注
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    说明

    Sequence similarities: Belongs to the CCN family.

    Tissue Specificity: Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .

    板类型
    Pre-coated
    实验流程
    human NOV ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NOV has been precoated onto 96-well plates. Standards(NSO, T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOV is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NOV amount of sample captured in plate.
    实验流程

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NOV standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NOV standard solution and each sample be measured in duplicate.

    实验精密度
    • Sample 1: n=16, Mean(pg/ml): 154, Standard deviation: 8.78, CV(%): 5.7
    • Sample 2: n=16, Mean(pg/ml): 653, Standard deviation: 40.5, CV(%): 6.2
    • Sample 3: n=16, Mean(pg/ml): 1286, Standard deviation: 61.73, CV(%): 4.8,
    • Sample 1: n=24, Mean(pg/ml): 197, Standard deviation: 12.61, CV(%): 6.4
    • Sample 2: n=24, Mean(pg/ml): 728, Standard deviation: 48.78, CV(%): 6.7
    • Sample 3: n=24, Mean(pg/ml): 1473, Standard deviation: 78.1, CV(%): 5.3
    限制
    仅限研究用
  • 注意事项
    Avoid multiple freeze-thaw cycles.
    储存条件
    -20 °C,4 °C
    储存方法
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    有效期
    12 months
  • 抗原 See all NOV ELISA试剂盒
    NOV (Nephroblastoma Overexpressed (NOV))
    别名
    NOV (NOV 产品)
    别名
    CCN3 ELISA Kit, IBP-9 ELISA Kit, IGFBP-9 ELISA Kit, IGFBP9 ELISA Kit, NOVh ELISA Kit, nov-A ELISA Kit, xnov ELISA Kit, NovH ELISA Kit, C130088N23Rik ELISA Kit, nephroblastoma overexpressed ELISA Kit, nephroblastoma overexpressed L homeolog ELISA Kit, nephroblastoma overexpressed gene ELISA Kit, NOV ELISA Kit, nov.L ELISA Kit, Nov ELISA Kit
    背景

    Protein Function: Immediate-early protein likely to play a role in cell growth regulation. .

    Background: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.

    Synonyms: Protein NOV homolog,NovH,CCN family member 3,Insulin-like growth factor-binding protein 9,IBP-9,IGF-binding protein 9,IGFBP-9,Nephroblastoma-overexpressed gene protein homolog,NOV,CCN3, IGFBP9, NOVH,

    Full Gene Name: Protein NOV homolog

    Cellular Localisation: Secreted.
    基因ID
    4856
    UniProt
    P48745
    途径
    Smooth Muscle Cell Migration, Growth Factor Binding
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