NOV ELISA 试剂盒
Quick Overview for NOV ELISA 试剂盒 (ABIN1889323)
抗原
See all NOV ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 31.2 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NOV/CCN3
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: T32-M357 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <2pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: T32-M357
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Nephroblastoma Overexpressed (NOV) ELISA Kit
NOV 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Nephroblastoma Overexpressed (NOV) ELISA KitNOV 适用: 小鼠 Colorimetric Sandwich ELISA 23.5 pg/mL - 1500 pg/mL Plasma, Serum, Tissue Homogenate
Nephroblastoma Overexpressed (NOV) ELISA KitNOV 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Nephroblastoma Overexpressed (NOV) ELISA KitNOV 适用: 小鼠 AA 22-354 Colorimetric Sandwich ELISA
Nephroblastoma Overexpressed (NOV) ELISA KitNOV 适用: 人 Colorimetric Sandwich ELISA 2 pg/mL-30 ng/mL Cell Culture Supernatant, Plasma, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the CCN family.
Tissue Specificity: Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type. .
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板类型
- Pre-coated
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实验流程
- human NOV ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NOV has been precoated onto 96-well plates. Standards(NSO, T32-M357) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NOV is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NOV amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human NOV standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NOV standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 154, Standard deviation: 8.78, CV(%): 5.7
- Sample 2: n=16, Mean(pg/ml): 653, Standard deviation: 40.5, CV(%): 6.2
- Sample 3: n=16, Mean(pg/ml): 1286, Standard deviation: 61.73, CV(%): 4.8,
- Sample 1: n=24, Mean(pg/ml): 197, Standard deviation: 12.61, CV(%): 6.4
- Sample 2: n=24, Mean(pg/ml): 728, Standard deviation: 48.78, CV(%): 6.7
- Sample 3: n=24, Mean(pg/ml): 1473, Standard deviation: 78.1, CV(%): 5.3
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- NOV (Nephroblastoma Overexpressed (NOV))
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别名
- NOV
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背景
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Protein Function: Immediate-early protein likely to play a role in cell growth regulation. .
Background: NOV(nephroblastoma overexpressed), also known as CCN3, is a matricellular protein that in humans is encoded by the NOV gene. The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. This gene is mapped to 8q24.12. NOV is a potentially useful marker for the diagnosis of adrenal gland diseases, malignant adrenocortical tumors, multiple sclerosis and so on. Moreover, reduced expression of NOV in ACTs may play an important role in the process of childhood ACT tumorigenesis. Though studying, it identified Nov as a regulator of human hematopoietic stem or progenitor cells.
Synonyms: Protein NOV homolog,NovH,CCN family member 3,Insulin-like growth factor-binding protein 9,IBP-9,IGF-binding protein 9,IGFBP-9,Nephroblastoma-overexpressed gene protein homolog,NOV,CCN3, IGFBP9, NOVH,
Full Gene Name: Protein NOV homolog
Cellular Localisation: Secreted. -
基因ID
- 4856
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UniProt
- P48745
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途径
- Smooth Muscle Cell Migration, Growth Factor Binding
抗原 See all NOV ELISA试剂盒
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