MST1 ELISA 试剂盒
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Quick Overview for MST1 ELISA 试剂盒 (ABIN1889321)
抗原
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit to quantitate Human MST1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NS0
Immunogen sequence: Q19-G711Capture antibody and Detection antibody: monoclonal antibody from mouse, monoclonal antibody from mouse
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA Kit
MST1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 大鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 pg/mL - 200 pg/mL Plasma, Serum
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Plasma, Serum
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 7.7%, 4%, 5.3%
Inter-Assay Precision (CV%): 9.6%, 5.3%, 6.6% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))
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别名
- MST1
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背景
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Background: Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
Gene Full Name: macrophage stimulating 1
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基因ID
- 4485
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UniProt
- P26927
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