MST1 ELISA 试剂盒
Quick Overview for MST1 ELISA 试剂盒 (ABIN1889321)
抗原
See all MST1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MSP/MST1
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: Q19-G711 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: Q19-G711
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Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA Kit
MST1 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 小鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum
Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1) ELISA KitMST1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.
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板类型
- Pre-coated
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实验流程
- human MSP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MSP has been precoated onto 96-well plates. Standards(NSO, Q19-G711) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MSP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human MSP amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human MSP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human MSP standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 1126, Standard deviation: 56.3, CV(%): 5
- Sample 2: n=16, Mean(pg/ml): 2738, Standard deviation: 170, CV(%): 6.2
- Sample 3: n=16, Mean(pg/ml): 5273, Standard deviation: 242.6, CV(%): 4.6,
- Sample 1: n=24, Mean(pg/ml): 1384, Standard deviation: 78.9, CV(%): 5.7
- Sample 2: n=24, Mean(pg/ml): 2941, Standard deviation: 206, CV(%): 7
- Sample 3: n=24, Mean(pg/ml): 5527, Standard deviation: 364.8, CV(%): 6.6
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- MST1 (Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like) (MST1))
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别名
- MST1
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背景
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Background: Macrophage Stimulating 1(SMT1), also known as Hepatocyte growth factor-like protein(HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3(3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.
Synonyms: Hepatocyte growth factor-like protein,Macrophage stimulatory protein,Macrophage-stimulating protein,MSP,Hepatocyte growth factor-like protein alpha chain,Hepatocyte growth factor-like protein beta chain,MST1,D3F15S2, DNF15S2, HGFL,
Full Gene Name: Hepatocyte growth factor-like protein
Cellular Localisation: Secreted. -
基因ID
- 4485
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UniProt
- P26927
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