XCL1 ELISA 试剂盒
Quick Overview for XCL1 ELISA 试剂盒 (ABIN1889320)
抗原
See all XCL1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 62.5 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human XCL1/Lymphotactin
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: V22-G114 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <1pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: E.coli
Immunogen sequence: V22-G114
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Chemokine (C Motif) Ligand 1 (XCL1) ELISA Kit
XCL1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 猴 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 AA 22-114 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 40-3000 pg/mL Cell Culture Supernatant, Plasma, Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 小鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C Motif) Ligand 1 (XCL1) ELISA KitXCL1 适用: 人 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the intercrine gamma family.
Tissue Specificity: Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.
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板类型
- Pre-coated
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实验流程
- human XCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for XCL1 has been precoated onto 96-well plates. Standards(E.coli, V22-G114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for XCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human XCL1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human XCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human XCL1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.045, CV(%): 4.2
- Sample 2: n=16, Mean(ng/ml): 2.12, Standard deviation: 0.076, CV(%): 3.6
- Sample 3: n=16, Mean(ng/ml): 3.85, Standard deviation: 0.2, CV(%): 5.2,
- Sample 1: n=24, Mean(ng/ml): 1.42, Standard deviation: 0.097, CV(%): 6.8
- Sample 2: n=24, Mean(ng/ml): 2.56, Standard deviation: 0.184, CV(%): 7.2
- Sample 3: n=24, Mean(ng/ml): 3.78, Standard deviation: 0.295, CV(%): 7.8
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- XCL1 (Chemokine (C Motif) Ligand 1 (XCL1))
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别名
- XCL1
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背景
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Protein Function: Chemotactic activity for lymphocytes but not for monocytes or neutrophils.
Background: Chemokine(C motif) ligand(XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.
Synonyms: Lymphotactin,ATAC,C motif chemokine 1,Cytokine SCM-1,Lymphotaxin,SCM-1-alpha,Small-inducible cytokine C1,XC chemokine ligand 1,XCL1,LTN, SCYC1,
Full Gene Name: Lymphotactin
Cellular Localisation: Secreted. -
基因ID
- 6375
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UniProt
- P47992
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途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原 See all XCL1 ELISA试剂盒
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