ESM1 ELISA 试剂盒
(1 reference)Quick Overview for ESM1 ELISA 试剂盒 (ABIN1889318)
抗原
See all ESM1 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 23.4 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse ESM1/Endocan
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: W20-R184 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: W20-R184
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Endothelial Cell-Specific Molecule 1 (ESM1) ELISA Kit
ESM1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 人 AA 20-184 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 人 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Endothelial Cell-Specific Molecule 1 (ESM1) ELISA KitESM1 适用: 小鼠 Colorimetric Sandwich ELISA 23.4 pg/mL - 1500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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板类型
- Pre-coated
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实验流程
- mouse ESM1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for ESM1 has been precoated onto 96-well plates. Standards(NSO, W20-R184) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ESM1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse ESM1 amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/mL, 46.8pg/mL, 23.4pg/mL mouse ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse ESM1 standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(pg/ml): 56, Standard deviation: 1.85, CV(%): 3.3
- Sample 2: n=16, Mean(pg/ml): 673, Standard deviation: 31.6, CV(%): 4.7
- Sample 3: n=16, Mean(pg/ml): 1033, Standard deviation: 57, CV(%): 5.5,
- Sample 1: n=24, Mean(pg/ml): 47, Standard deviation: 1.93, CV(%): 4.1
- Sample 2: n=24, Mean(pg/ml): 660, Standard deviation: 34.98, CV(%): 5.3
- Sample 3: n=24, Mean(pg/ml): 1135, Standard deviation: 72.64, CV(%): 6.4
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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: "ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma." in: Oncotarget, Vol. 7, Issue 43, pp. 70738-70749, (2018) (PubMed).
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: "ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma." in: Oncotarget, Vol. 7, Issue 43, pp. 70738-70749, (2018) (PubMed).
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- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
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别名
- ESM1
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背景
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Protein Function: Involved in angiogenesis, promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions (By similarity). .
Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM-1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2(fibroblast growth factor 2).
Synonyms: Endothelial cell-specific molecule 1,ESM-1,Esm1,
Full Gene Name: Endothelial cell-specific molecule 1
Cellular Localisation: Secreted. -
基因ID
- 71690
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UniProt
- Q9QYY7
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途径
- Growth Factor Binding
抗原 See all ESM1 ELISA试剂盒
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