IL7R ELISA 试剂盒
Quick Overview for IL7R ELISA 试剂盒 (ABIN1889315)
抗原
See all IL7R ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 312 pg/mL
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原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL7R/CD127
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品牌
- PicoKine™
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: NSO
Immunogen sequence: E21-I262 -
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- <10pg/mL
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试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
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免疫原
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Expression system for standard: NSO
Immunogen sequence: E21-I262
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Interleukin 7 Receptor (IL7R) ELISA Kit
IL7R 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Interleukin 7 Receptor (IL7R) ELISA KitIL7R 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Interleukin 7 Receptor (IL7R) ELISA KitIL7R 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Interleukin 7 Receptor (IL7R) ELISA KitIL7R 适用: 小鼠 AA 21-239 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Serum
Interleukin 7 Receptor (IL7R) ELISA KitIL7R 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Serum
Interleukin 7 Receptor (IL7R) ELISA KitIL7R 适用: 小鼠 Colorimetric Sandwich ELISA 312 pg/mL - 20000 pg/mL Cell Culture Supernatant, Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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说明
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Sequence similarities: Belongs to the type I cytokine receptor family. Type 4 subfamily.
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板类型
- Pre-coated
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实验流程
- human IL7R ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL7R has been precoated onto 96-well plates. Standards(NSO, E21-I262) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL7R is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL7R amount of sample captured in plate.
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实验流程
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Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human IL7R standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IL7R standard solution and each sample be measured in duplicate.
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实验精密度
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- Sample 1: n=16, Mean(ng/ml): 2.8, Standard deviation: 0.168, CV(%): 6
- Sample 2: n=16, Mean(ng/ml): 8, Standard deviation: 0.36, CV(%): 4.5
- Sample 3: n=16, Mean(ng/ml): 14.3, Standard deviation: 0.815, CV(%): 5.7,
- Sample 1: n=24, Mean(ng/ml): 3.4, Standard deviation: 0.245, CV(%): 7.2
- Sample 2: n=24, Mean(ng/ml): 9.1, Standard deviation: 0.51, CV(%): 5.6
- Sample 3: n=24, Mean(ng/ml): 16.2, Standard deviation: 1.07, CV(%): 6.6
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限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- -20 °C,4 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
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有效期
- 12 months
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- IL7R (Interleukin 7 Receptor (IL7R))
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别名
- IL7R
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背景
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Protein Function: Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
Background: The interleukin-7 receptor(IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7(IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain(IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V(D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
Synonyms: Interleukin-7 receptor subunit alpha,IL-7 receptor subunit alpha,IL-7R subunit alpha,IL-7R-alpha,IL-7RA,CDw127,CD127,IL7R,
Full Gene Name: Interleukin-7 receptor subunit alpha
Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. -
基因ID
- 3575
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UniProt
- P16871
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
抗原 See all IL7R ELISA试剂盒
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