CCL28 ELISA 试剂盒
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- 抗原 See all CCL28 ELISA试剂盒
- CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
- 抗原表位
- AA 23-130
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适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 31.2-2000 pg/mL
- 最低检测浓度
- 31.2 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL28
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
- Analytical Method
- Quantitative
- 特异性
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Expression system for standard: E.coli
Immunogen sequence: I23-R130 - 交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
- 灵敏度
- <10pg/mL
- 试剂未包括
- Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
- 免疫原
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Expression system for standard: E.coli
Immunogen sequence: I23-R130 - Featured
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Chemokine (C-C Motif) Ligand 28 (CCL28) ELISA Kit
CCL28 适用: 小鼠 Colorimetric Sandwich ELISA 37.5 pg/mL - 2400 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 28 (CCL28) ELISA KitCCL28 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 28 (CCL28) ELISA KitCCL28 适用: 人 AA 23-127 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 28 (CCL28) ELISA KitCCL28 适用: 小鼠 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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- 应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
- 说明
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Tissue Specificity: Mainly expressed in testis, epithelial cells of normal colon, kidney, Peyer patches, lymph nodes. Also found in lower levels in brain, spleen and lung.
- 板类型
- Pre-coated
- 实验流程
- mouse CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL28 has been precoated onto 96-well plates. Standards(E.coli, I23-R130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL28 amount of sample captured in plate.
- 实验流程
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Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL28 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CCL28 standard solution and each sample be measured in duplicate.
- 实验精密度
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- Sample 1: n=16, Mean(pg/ml): 68, Standard deviation: 4.89, CV(%): 7.2
- Sample 2: n=16, Mean(pg/ml): 272, Standard deviation: 17.14, CV(%): 6.3
- Sample 3: n=16, Mean(pg/ml): 1013, Standard deviation: 38.49, CV(%): 3.8,
- Sample 1: n=24, Mean(pg/ml): 71, Standard deviation: 6.32, CV(%): 8.9
- Sample 2: n=24, Mean(pg/ml): 283, Standard deviation: 21.51, CV(%): 7.6
- Sample 3: n=24, Mean(pg/ml): 1154, Standard deviation: 83.09, CV(%): 7.2
- 限制
- 仅限研究用
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- 注意事项
- Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C,4 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
- 有效期
- 12 months
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- 抗原 See all CCL28 ELISA试剂盒
- CCL28 (Chemokine (C-C Motif) Ligand 28 (CCL28))
- 别名
- CCL28 (CCL28 产品)
- 别名
- Scya28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, nrpb ELISA Kit, enc-1 ELISA Kit, pig10 ELISA Kit, Encr-3 ELISA Kit, klhl35 ELISA Kit, Xencr-3 ELISA Kit, tp53i10 ELISA Kit, LOC100303767 ELISA Kit, CCK1 ELISA Kit, MEC ELISA Kit, SCYA28 ELISA Kit, C-C motif chemokine ligand 28 ELISA Kit, ectodermal-neural cortex 1 (with BTB domain) ELISA Kit, C-C motif chemokine 28 ELISA Kit, chemokine (C-C motif) ligand 28 ELISA Kit, Ccl28 ELISA Kit, CCL28 ELISA Kit, ccl28 ELISA Kit, enc1 ELISA Kit, LOC100303767 ELISA Kit
- 背景
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Protein Function: Chemotactic for resting CD4, CD8 T-cells and eosinophils (By similarity). Binds to CCR10 and induces calcium mobilization in a dose-dependent manner. .
Background: CCL28, also known as mucosae-associated epithelial chemokine(MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T(Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Synonyms: C-C motif chemokine 28,Small-inducible cytokine A28,Ccl28,Scya28,
Full Gene Name: C-C motif chemokine 28
Cellular Localisation: Secreted. - 基因ID
- 56838
- UniProt
- Q9JIL2
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